ADIAPURE™

ADIAPURE™ DNA extraction methods 

ADIAVET^TM kits contain only amplification PCR reagents. In order to achieve very good performances on a wide range of samples, we recommend and validate many different DNA/RNA global extraction methods. It is very useful to stay flexible but without decreasing any performances (sensitivity, inhibition rate).

Today DNA and RNA extracts usable with ADIAVET^TM kits can be prepared with :

Different technologies of purification : silica membrane, magnetic beads, ultra-filtration

Different automated system : centrifugation, vacuum, magnetic

For different throughputs : from individual analysis to 96 samples extraction per run

For some specific applications we propose our own extraction method with ADIAPURE^TM range in combination with ADIAVET^TM PCR kits :

adiapurekits.jpg

Show in media library: 

• ADIAPURE^TM purification kit: for DNA extraction of Avian mycoplasmas and Ornithobacterium rhinotracheale from swabs
• ADIAPURE^TM milk kit: for DNA extraction of Mycobacterium avium subsp. paratuberculosis from dairy products 
• ADIAPURE^TM TLB buffer: single lysis buffer for RNA extraction of BVD virus from ear notches samples

Samples preparation steps (individual or pool) are developed and validated to be adapted to different epidemiological situations : diagnostic, individual analysis, screening with pool of samples or environmental samples.

ADIAPURE™ kits :

• ADIAPURE^TM PURIFICATION KIT

• ADIAPURE^TM MILK KIT

• ADIAPURE^TM TLB BUFFER

Find out more details on the ADIAPURE™ DNA purification range on the technical details tab.

Why choose ADIAPURE™ for DNA purification in veterinary diagnostic?

PERFORMANCE : ADIAPURE™ kits are fully validated with ADIAVET^TM Real Time PCR kits and improve their performances (level of inhibition, sensitivity)

EASY TO USE : ADIAPURE^TM protocols are simple and adapted to a routine buzzy diagnostic laboratory

HIGH THROUGHPUT : Direct lysis method or 96 wells-plate purification system allow to treat a lot of samples all together

DNA/RNA extraction step

In order to achieve very good performances on a wide range of samples, we recommend and validate many different DNA/RNA global extraction methods. It is very useful to stay flexible but without decreasing any performances (sensitivity, inhibition rate).

In this objective also, ADIAPURE^TM protocols have been developed for some specific veterinary applications where other common DNA extraction methods didn’t give expected results in term of performances and/or throughput.

Kits include all components for DNA extraction and/or purification.

Find out more details on equipment requested for using ADIAPURE™ DNA purification range on the option & accessories tab.

AVIAN DIAGNOSTIC

ADIAPURE™ PURIFICATION KIT

RUMINANTS DIAGNOSTIC

ADIAPURE™ MILK KIT

ADIAPURE TLB™ BUFFER

For using ADIAPURE^TM purification kit

• A manifold for 96-wells plates (eg : PALL). 

pall.jpg

Show in media library: 

• A vacuum pump adapted to manifold (0.9 mbar pressure minimum). (eg : KNF Series N 035.3 A_.18)

pump.jpg

Show in media library: 

For using ADIAPURE^TM MILK kit

• A magnet for 15mL/50ML tubes (eg : Qiagen, 36935)

magnet.jpg

Show in media library: 

• A rotating homogenizer (eg : Stuart,SB3)

homogenizer.jpg

Show in media library: